抗体作製
Monoclonal Antibody
大川研では、エピゲノム解析に必要な各種転写因子（主に骨格筋分化）、ヒストン、RNAポリメラーゼに対するモノクローナル抗体の開発を行っています。
これら抗体は、試用であれば提供、一定量以上の場合は共同研究ベースで供与いたします。
必要な方はお気軽にメールください。E-mail
We have developed monoclonal antibodies against various transcription factors (mainly skeletal muscle differentiation), histones, and RNA polymerase necessary for epigenome analysis.
These antibodies are provided for trial use, and for fixed doses or more on a joint research basis.
Please feel free to email me if you need one.E-mail
Rat anti-C/EBPβ Mab
Production of a monoclonal antibody for C/EBPβ: the subnuclear localization of C/EBPβ in mouse L929 cells.
Harada A, Okazaki E, Okada S, Tachibana T, Ohkawa Y.
Monoclonal antibodies in immunodiagnosis and immunotherapy 2014 Feb;33(1):34-7.Rat anti-phosphorylated histone H3 (S10, T11, S28, S31, and T32) Mab
A panel of specific monoclonal antibodies directed against various phosphorylated histones H3.
Yoshimi T, Ohkawa Y, Azuma M, Tachibana T.
Monoclonal antibodies in immunodiagnosis and immunotherapy 2013 Apr;32(2):119-24.Rat anti-H3.3 Mab, Mouse anti-H3.1/2 Mab
Chd2 interacts with H3.3 to determine myogenic cell fate.
Harada A, Okada S, Konno D, Odawara J, Yoshimi T, Yoshimura S, Kumamaru H, Saiwai H, Tsubota T, Kurumizaka H, Akashi K, Tachibana T, Imbalzano AN, Ohkawa Y.
EMBO J. 2012 Jun 29;31(13):2994-3007. doi: 10.1038/emboj.2012.136. Epub 2012 May 8.Rat anti-phosphorylated RNAPII (S2, S5, S5+S2, S7) Mab,
The classification of mRNA expression levels by the phosphorylation state of RNAPII CTD based on a combined genome-wide approach.
Odawara J, Harada A, Yoshimi T, Maehara K, Tachibana T, Okada S, Akashi K, Ohkawa Y.
BMC Genomics. 2011 Oct 20;12:516. doi: 10.1186/1471-2164-12-516.Rat anti-hsp72 Mab
Generation of a rat monoclonal antibody specific for hsp72.
Tanaka M, Shiota M, Okada S, Harada A, Odawara J, Mun S, Iwao H, Ohkawa Y.
Hybridoma (Larchmt). 2011 Aug;30(4):397-400.Rat anti-Hsc70 Mab
Generation of a rat monoclonal antibody specific for heat shock cognate protein 70.
Shiota M, Saiwai H, Mun S, Harada A, Okada S, Odawara J, Tanaka M, Iwao H, Ohkawa Y.
Hybridoma (Larchmt). 2010 Oct;29(5):453-6.Rat anti-Dhx9 Mab
Monoclonal antibody specific for Dhx9/NDHII/RHA.
Kotani M, Harada A, Odawara J, Azuma M, Okada S, Nishiyama Y, Nakamura M, Tachibana T, Ohkawa Y.
Hybridoma (Larchmt) 2010 Jun;29(3):259-61.Rat anti-MyoD Mab
Rat monoclonal antibody specific for MyoD.
Harada A, Ohkawa Y, Ao S, Odawara J, Okada S, Azuma M, Nishiyama Y, Nakamura M, Tachibana T.
Hybridoma (Larchmt). 2010 Jun;29(3):255-8.Rat anti-Chd1 Mab
Rat monoclonal antibody specific for the chromatin remodeling factor, CHD1.
Yoshimura S, Harada A, Odawara J, Azuma M, Okada S, Nakamura M, Ohkawa Y, Tachibana T.
Hybridoma (Larchmt). 2010 Jun;29(3):237-40.Rat anti-Chd2 Mab
Generation of a rat monoclonal antibody specific for CHD2.
Harada A, Yoshimura S, Odawara J, Azuma M, Okada S, Nakamura M, Tachibana T, Ohkawa Y.
Hybridoma (Larchmt). 2010 Apr;29(2):173-7.Rat anti-Chd5 Mab
A rat monoclonal antibody against the chromatin remodeling factor CHD5.
Yoshimura S, Yoshimi T, Ohkawa Y, Azuma M, Tachibana T.
Hybridoma (Larchmt). 2010 Feb;29(1):63-6. doi: 10.1089/hyb.2009.0069.Rat anti-Myf5 Mab
Production of a rat monoclonal antibody specific for Myf5.
Harada A, Okada S, Odawara J, Kumamaru H, Saiwai H, Aoki M, Nakamura M, Nishiyama Y, Ohkawa Y.
Hybridoma (Larchmt). 2010 Feb;29(1):59-62. doi: 10.1089/hyb.2009.0066.Rat anti-Brg1 Mab
Production of a rat monoclonal antibody against Brg1.
Ohkawa Y, Harada A, Nakamura M, Yoshimura S, Tachibana T.
Hybridoma (Larchmt). 2009 Dec;28(6):463-6. doi: 10.1089/hyb.2009.0041.Rat anti-Brm Mab
Generation of a rat monoclonal antibody specific for Brm.
Okada S, Harada A, Saiwai H, Nakamura M, Ohkawa Y.
Hybridoma (Larchmt). 2009 Dec;28(6):455-8. doi: 10.1089/hyb.2009.0044.Rat anti-Pax7 Mab
Generation of a rat monoclonal antibody specific for Pax7.
Harada A, Okada S, Saiwai H, Aoki M, Nakamura M, Ohkawa Y.
Hybridoma (Larchmt). 2009 Dec;28(6):451-3. doi: 10.1089/hyb.2009.0039.
エピゲノム解析
Epigenome Profilling Technology
ChIL-Seq
少数細胞エピゲノム解析に適した手法、クロマチン挿入標識法を開発して公開しています。細胞・組織染色ベースでChIPseqと同様データを得ることが可能で、単一細胞レベルの解析にも成功しています。本技術を用いた共同研究の提案はこちらにメールください。E-mail
We have developed and released chromatin insertion labeling method suitable for minority cell epigenome analysis. It is possible to obtain data similar to ChIPseq on a cell / tissue staining basis, and has also succeeded in single cell level analysis. Please email here for collaborative research using this technology.E-mail
The Original Paper [Journal Site]
The detailed protocol for ChIL-Seq [Nature Protocol Exchange]
Movie is Comming soon.
Flow cytometric sorting of cell nuclei from tissues.
神経、骨格筋等の組織から核を単離し、転写因子等を標識してFACSにより分画する手法です。単離核はChIP, RT-PCR等に利用できます。
This method could isolates nuclei from tissues such as nerves and skeletal muscles, and isolated nuclei could be labeled with anti-nuclei-protein antibody(transcription factor, histone modification, etc) for separating by FACS. FACS-sorted nuclei can be used for ChIP, RT-PCR, etc.
The detailed protocol for Nuclei Sorting [Pubmed]
Restriction Enzyme Accessibility Assay
PCRで検出可能なクロマチン弛緩度(ATACseq, DNaseI Hypersensitivity Assay等々で測定される）の測定手法です。
The Original Paper [Pubmed]
The detailed protocol for Restriction Enzyme Accessibility Assay [Methods in Molecular Biology]
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